A protein important for cognition and storing long-term memories looks and acts like a protein from viruses. The protein, called Arc, has properties similar to those that viruses use for infecting host cells, and originated from a chance evolutionary event that occurred hundreds of millions of years ago.
The prospect that virus-like proteins could be the basis for a novel form of cell-to-cell communication in the brain could change our understanding of how memories are made, according to Jason Shepherd, Ph.D., a neuroscientist at University of Utah Health and senior author of the study.
Shepherd first suspected that something was different about Arc when his colleagues captured an image of the protein showing that Arc was assembling into large structures. With a shape that resembles a capsule from a lunar lander, these structures looked a lot like the retrovirus, HIV.
At the time, we did not know much about the molecular function or evolutionary history of Arc, said Shepherd who has researched the protein for 15 years.
“I had almost lost interest in the protein, to be honest. After seeing the capsids, we knew we were onto something interesting.”
The gap in research was not for want of an interesting subject. Prior work had shown that mice lacking Arc forgot things they had learned a mere 24 hours earlier.
[caption id=“attachment_94120” align=“alignright” width=“350”] Credit: Elissa Pastuzyn[/caption]
Further, their brains lacked plasticity. There is a window of time early in life when the brain is like a sponge, easily soaking up new knowledge and skills.
Without Arc, the window never opens.
Scientists had never considered that mechanisms responsible for acquiring knowledge could stem from foreign origins. Now, the work by Shepherd and his team has raised this intriguing possibility.
New Set Of Eyes
Seeing Arc’s unusual propensity to form virus-like structures prompted Shepherd to scrutinize the protein sequence with a new set of eyes.
He found that regions of the code were similar to that from viral capsids. An essential tool for viral infection, capsids carry virus’ genetic information and deliver it from cell to cell in its victim.
[caption id=“attachment_94119” align=“aligncenter” width=“680”] Credit: University of Utah Health[/caption]
Given that Arc looks like a viral protein, Shepherd and his colleagues designed a set of experiments to test whether it also acts like one.
They first determined that several copies of Arc self-assemble into hollow virus-like capsids and stash its own genetic material, in this case mRNA, inside them. When the scientists added the capsids to mouse brain cells, or neurons, growing in a dish, Arc transferred its genetic cargo into the cells.
Cell-to-cell RNA Transport
After viruses invade host cells, they emerge ready to infect once again. It appears that Arc works in a similar way.
The scientists gathered Arc that had been released from mouse neurons and determined that the proteins and their cargo could be taken up by another set of neurons. Unlike for viruses, activating neurons mobilizes Arc, triggering the release of capsids.
“We went into this line of research knowing that Arc was special in many ways, but when we discovered that Arc was able to mediate cell-to-cell transport of RNA, we were floored. No other non-viral protein that we know of acts in this way,”
said the study’s lead author, postdoctoral fellow Elissa Pastuzyn, Ph.D.
Lightning Strikes Twice
The story of Arc’s origin is relayed through the genomes of animals throughout evolutionary time. 350-400 million years ago, a chance occurrence struck four-limbed creatures that roamed the earth.
An ancestor to retroviruses, called retrotransposons, inserted its genetic material into the animals’ DNA. The event led to the mammalian Arc that we know today.
The significance of such an event is hinted at by the fact that it happened more than once.
An accompanying paper in the same issue of Cell shows that a version of Arc found in flies also looks and acts like a viral capsid. Vivian Budnik’s lab at the University of Massachusetts shows that fly Arc transports RNA from neurons to muscles to control movement.
Even though mammalian and fly Arc evolved from the same class of retrotransposons, the event in flies occurred about 150 million years later.
“As an evolutionary biologist this is what is the most exciting to me. The fact that it happened at least twice makes us think that it happened even more,”
said co-author Cédric Feschotte, Ph.D., a professor at Cornell University.
Shepherd believes this could mean that it is advantageous to have this viral-inspired system in place, and it may represent a novel form of intercellular communication. This hypothesis remains to be tested in mammals.
“Knowing what cargo Arc vesicles transport in living animals will be critical to understanding the function of this pathway,” he said.
Remember the unusual viral-like protein that you just learned about? It could be controlling your memory.
Pastuzyn, Elissa D. et al. The Neuronal Gene Arc Encodes a Repurposed Retrotransposon Gag Protein that Mediates Intercellular RNA Transfer Cell, Volume 172 , Issue 1 , 275 - 288.e18
Top Image: Arc can encapsulates genetic material (polyhedron enveloping the ribbon-like strands) and delivers it to brain cells in a manner similar to the way in which viruses infect host cells. Credit: Chris Manfre.