Boosting mitochondrial defences against a particular form of protein stress, enables them to not only protect themselves, but to also reduce the formation of amyloid plaques, new research from Ecole Polytechnique Federale de Lausanne has found.
Alzheimer’s disease is the most common form of dementia and neurodegeneration worldwide. A major hallmark of the disease is the accumulation of toxic plaques in the brain, formed by the abnormal aggregation of a protein called beta-amyloid inside neurons.
Still without cure, Alzheimer’s poses a significant burden on public health systems. Most treatments focus on reducing the formation of amyloid plaques, but these approaches have been inconclusive. As a result, scientists are now searching for alternative treatment strategies, one of which is to consider Alzheimer’s as a metabolic disease.
Central To Metabolism
During normal aging and age-associated diseases such as Alzheimer’s, cells face increasing damage and struggle to protect and replace dysfunctional mitochondria. Since mitochondria provide energy to brain cells, leaving them unprotected in Alzheimer’s disease favors brain damage, giving rise to symptoms like memory loss over the years.
The scientists identified two mechanisms that control the quality of mitochondria.
First, the “mitochondrial unfolded protein response” (UPRmt), which protects mitochondria from stress stimuli. Second, mitophagy, a process that recycles defective mitochondria. Both these mechanisms are the key to delaying or preventing excessive mitochondrial damage during disease.
While we have known for a while that mitochondria are dysfunctional in the brains of Alzheimer’s patients, this is the first evidence that they actually try to fight the disease by boosting quality control pathways.
“These defence and recycle pathways of the mitochondria are essential in organisms, from the worm C. elegans all the way to humans. So we decided to pharmacologically activate them,"
first author Vincenzo Sorrentino said.
Mitochondrial Defence Pathways
The team started by testing well-established compounds, such as the antibiotic doxycycline and the vitamin nicotinamide riboside (NR), which can turn on the UPRmt and mitophagy defense systems in a worm model (C. elegans) of Alzheimer’s disease.
The health, performance and lifespan of worms exposed to the drugs increased remarkably compared with untreated worms. Plaque formation was also significantly reduced in the treated animals.
And most significantly, the scientists observed similar improvements when they turned on the same mitochondrial defence pathways in cultured human neuronal cells, using the same drugs.
The encouraging results led the researchers to test NR in a mouse model of Alzheimer’s disease. Just like C. elegans, the mice saw a significant improvement of mitochondrial function and a reduction in the number of amyloid plaques.
But most importantly, the scientists observed a striking normalization of the cognitive function in the mice. This has tremendous implications from a clinical perspective.
Novel Therapeutic Approach
According to Johan Auwerx, tackling Alzheimer’s through mitochondria could make all the difference.
“So far, Alzheimer’s disease has been considered to be mostly the consequence of the accumulation of amyloid plaques in the brain,” he says. “We have shown that restoring mitochondrial health reduces plaque formation - but, above all, it also improves brain function, which is the ultimate objective of all Alzheimer’s researchers and patients."
The strategy represents a novel therapeutic approach to slow down the progression of neurodegeneration in Alzheimer’s disease, and possibly even in other disorders such as Parkinson’s disease, which is also characterized by profound mitochondrial and metabolic defects.
The approach remains to be tested in human patients.
“By targeting mitochondria, NR and other molecules that stimulate their ‘defence and recycle’ systems could perhaps succeed where so many drugs, most of which aim to decrease amyloid plaque formation, have failed,"
says Vincenzo Sorrentino.
Vincenzo Sorrentino, Mario Romani, Laurent Mouchiroud, John S. Beck, Hongbo Zhang, Davide D’ Amico, Norman Moullan, Francesca Potenza, Adrien W. Schmid, Solène Rietsch, Scott E. Counts & Johan Auwerx Enhancing mitochondrial proteostasis reduces amyloid-β proteotoxicity Nature doi:10.1038/nature25143
Image: Vincenzo Sorrentino, Mario Romani, Francesca Potenza/EPFL