Researchers have custom built two molecules which bind to and inhibit enzymes called tankyrases that are involved in an important cellular process called Wnt/β-catenin signalling.

The research team from Cancer Research at University of Bath, working with colleagues at the University of Oulu in Finland, hope these new molecules could one day become drugs for both cancer and diabetes.

The compounds are able to increase glucose uptake into fat cells and could help diabetes patients manage their disease. They also reduce the proliferation of colon cancer cells, and could be used to reveal the link between diabetes and cancer, which is poorly understood.

Wnt/β-catenin Signalling

High levels of Wnt/β-catenin signalling is observed in several cancers, in particular colon cancer, as well as many inflammatory diseases including chronic obstructive pulmonary disease and idiopathic pulmonary fibrosis. In laboratory experiments their custom built tankyrase inhibitors significantly reduced Wnt/β-catenin signalling and slowed down proliferation of colon cancer cells.

Another significant result from their research showed that targeting tankyrases results in increased uptake of glucose into fat cells.

Potent and selective inhibition of tankyrases could be useful for patients with Type II diabetes to help manage their disease, and possibly reduce the amount of insulin administered to patients with Type I diabetes, as high levels of insulin can interfere with other cellular pathways resulting to harmful effects.

Dr Amit Nathubhai, from the University of Bath’s Department of Pharmacy and Pharmacology, said:

“It’s early days but our new set of molecules are a great starting point towards the development of new drugs that target the tankyrases and Wnt signalling to treat cancer, diabetes and inflammatory diseases. We have shown they are potent and selective and we see enhanced glucose uptake and a marked reduction of colon cancer cell proliferation using our molecules.

“The next phase of our research is to further optimise our molecules and evaluate them using models of particular cancers and fibrotic diseases to reveal potential opportunities to develop new therapies.

In addition, we are investigating the effects of our tankyrase inhibitors in other biological models of diabetes and obesity. We hope to reveal the underlying mechanism(s) that link cancer, fibrosis, diabetes and obesity, which remains unclear."

Tankyrase Biomolecular Mechanisms

The research team believes that better understanding of tankyrases and Wnt signalling could unravel the biomolecular mechanisms that link obesity, diabetes and some cancers.

They plan to develop 3-D models using cells from patients with diseases such as colon cancer, prostate cancer, chronic obstructive pulmonary disease and idiopathic pulmonary fibrosis to better understand the effects their Tankyrase/Wnt signalling inhibitors may have on these conditions. They are also using their molecules to investigate reduction in fat and increased insulin sensitivity in other biological models of obesity and diabetes.

There are 5,000 new cases of idiopathic pulmonary fibrosis every year in the UK and 50% of patients die within three years of diagnosis. New treatments to combat such diseases are required and will significantly reduce the amount of lung transplants required.

By 2025, it is estimated that five million people will have diabetes in the UK which is equivalent to 400 new cases every day or 17 people every hour. Globally, it is estimated that 175 million people have undiagnosed Type 2 diabetes which can lead to obesity, cardiovascular disease, kidney disease, eye disease, amputation, depression, neuropathy, sexual dysfunction, complications in pregnancy and dementia.

Cancers that affect the digestive system such as colon cancer, bowel cancer and gastric cancer are the UK’s second biggest killing type of cancer. Treatment often involves surgery followed by chemotherapy.

Amit Nathubhai et al. Highly Potent and Isoform Selective Dual Site Binding Tankyrase/Wnt Signaling Inhibitors That Increase Cellular Glucose Uptake and Have Antiproliferative Activity Journal of Medicinal Chemistry (2017). DOI: 10.1021/acs.jmedchem.6b01574

Image: Dr Lari Lehtiö

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