Striatal Neurons Require Huntingtin For Survival In Aging

Neurons in the striatum, a brain area involved in controlling movement, require the huntingtin gene for regulating the body’s movements, maintaining cell health during aging, and developing functioning connections between cells, according to new work from a Duke University research team.

Addressing the gene’s role in maintaining those neural connections may provide a new avenue against Huntington’s, the researchers said.

Huntington’s Disease is an inherited neurodegenerative disorder that usually emerges in mid-life and leads to impaired motor control, dementia, and psychiatric symptoms. While the genetic basis of this lethal disease was identified more than two decades ago, there are no approved treatments yet to slow its progression or cure it.

Toxic To Neurons

Huntington’s is caused by a mutation in one of a person’s two copies of the huntingtin gene. The mutation results in the production of an aberrant version of the huntingtin protein, which is toxic to neurons.

Although the mutant protein is expressed throughout the body, neurons of the striatum are specifically vulnerable to its effects and degenerate as the disease progresses.

While the mutant huntingtin protein is damaging to neurons, it may also interfere with the remaining, non-mutated huntingtin’s ability to perform its normal functions.

Movement Regulation

Drugs currently being tested in clinical trials are designed to block the defective huntingtin protein, but they also end up decreasing the amount of normal huntingtin in neurons. Huntingtin is known to play several important functions in cells, but its specific role in striatal neuron health and function was not known.

“We hypothesized that the normal huntingtin gene plays a critical role in neuronal health and connectivity, and we wanted to determine what happens to striatal neurons that have had huntingtin eliminated,”

said lead author Cagla Eroglu, an associate professor of cell biology and neurobiology and the co-director of Regeneration Next Initiative at Duke.

The team found that deleting the huntingtin gene specifically from the striatal neurons of very young mice caused these neurons to die as the mice aged, similar to the pattern of neuron death seen in Huntington’s Disease.

striatal neurons

Credit: Caley Burrus, Duke University

They also found that mice lacking huntingtin in their striatal neurons were impaired in their ability to control their movement. Importantly, this loss of movement regulation happened even before the neurons themselves started to die.

“These findings suggest that cell death itself might not be the only trigger of Huntington’s Disease symptoms,”

Eroglu said.

Synaptic Connectivity

In a healthy brain, striatal neurons control movement by communicating with other neurons through connections called synapses. The researchers found that striatal neurons lacking huntingtin formed abnormal synaptic connections, which could potentially explain the problematic motor function of the mice.

“We believe changes at the neuronal and synapse level happening before cell death are contributing to the progression of the disease,”

said Caley Burrus, a Ph.D. candidate in Eroglu’s lab and first author of the study.

It’s possible, the authors said, that therapies to address the faults at the level of the synapse may be beneficial for patients. The team is continuing the research by investigating the precise role huntingtin plays in synapse development, which might lead to specific drugs designed to target these deficits.

“Understanding the critical roles huntingtin plays in neurons is essential to designing therapies that will help individuals with Huntington’s Disease in the safest and most effective way possible,”

Eroglu said.

[1] Caley J. Burrus, Spencer U. McKinstry, Namsoo Kim, M. Ilcim Ozlu, Aditya V. Santoki, Francia Y. Fang, Annie Ma, Yonca B. Karadeniz, Atesh K. Worthington, Ioannis Dragatsis, Scott Zeitlin, Henry H. Yin, Cagla Eroglu. Striatal Projection Neurons Require Huntingtin for Synaptic Connectivity and Survival. Cell Reports; Volume 30, Issue 3, p642-657.E6, Jan 21, 2020

Top Image: Loss of huntingtin protein in striatal neurons (red) causes neuron loss and an inflammatory response, shown by the infiltration of glial astrocytes (cyan). Credit: Caley Burrus, Duke University