Brown University researchers report that by imitating a natural process of cells, they have prevented the formation of protein clumps associated with frontotemporal dementia and amyotrophic lateral sclerosis (ALS) — also known as Lou Gehrig’s disease.
In lab cultures of human and yeast cells, the scientists stopped the harmful clumping of FUS proteins by exposing them to phosphorylation, a process that makes precise changes to the amino acid building blocks of proteins, increasing their negative electric charge. The research shows that the increase in charge causes the proteins to repel when they normally might aggregate.
The findings could eventually have positive implications for the treatment of ALS and dementia.
“No one has shown that you can use charge, and phosphorylation as a way to get charge, to disrupt these ALS-associated protein aggregates,”
says co-corresponding author Nicolas Fawzi, an assistant professor in the molecular pharmacology, physiology, and biotechnology department at Brown University.
Phosphorylation occurs in cells throughout the body for various reasons. Cells perform it on fused in sarcoma (FUS) proteins as part of a sequence of events related to DNA damage.
In the new study, the researchers examined both natural phosphorylation and artificial phosphorylation by replacing specific amino acids in FUS proteins.
He and co-lead author Veronica Ryan, a neuroscience graduate student, wanted to explore how and whether phosphorylation would affect the way FUS proteins can clump together, which undermines the health of neurons.
The first task was to identify several specific amino acid sites in the region FUS uses to bind with other proteins where the natural enzyme DNA-dependent protein kinase (DNA-PK) causes phosphorylation.
Their collaborators at the Uniformed Services University, including co-lead author Zachary Monahan and co-corresponding author Frank Shewmaker, continued the work by confirming these and more phosphorylation sites in FUS derived from human cells.
DNA-dependent Protein Kinase
With more than a dozen sites identified, the team then set out to imitate the work of DNA-PK in living cell cultures. Monahan and Shewmaker’s team engineered human FUS into brewer’s yeast and also implemented the phosphorylation mimicry in a culture of human cells.
What they observed in both cultures is that by increasing the degree of phosphorylation — from just two sites all the way up to 12 — they could proportionally reduce the propensity of the protein to aggregate into clumps.
In the yeast cells, they showed that the more effectively they prevented full aggregation — by adding more sites mimicking phosphorylation — the more robustly colonies of the cells would grow. That finding demonstrated that FUS clumping is toxic to cells.
The researchers showed how phosphorylation disrupts contacts between molecules of FUS. Meanwhile, computational modeling of the effect of the phosphorylation in the structure of the protein showed that the change in electric charge is what led to the difference in the proteins’ likelihood of aggregating.
New Hypotheses And Angles
Fawzi acknowledges that the approach the scientists used in the lab would not yet constitute a practical therapy for neurodegenerative diseases, but he says the demonstration that phosphorylation can be used to disrupt protein aggregation should motivate work to produce a treatment.
“There’s no therapy or cure for ALS and frontotemporal dementia,” Fawzi says. “What we need are new hypotheses and new angles.”
Fawzi hypothesizes that because phosphorylation is a natural process of cells, the key may be finding a “switch” to turn it on when needed. He also notes that many pharmaceutical companies have very active research groups dedicated to harnessing the phosphorylating enzymes known as kinases like DNA-PK.
A future therapy, he says, would have to be targeted — localized in the brain in the case of frontotemporal dementia, or focused on motor neurons in the case of ALS. Phosphorylating FUS in the body more generally could be harmful, given that it is presumed to have important roles in helping cells process RNA and may have a role in repairing DNA.
For now, the collaboration is focused on testing phosphorylation specifically in a model of a neurodegenerative disease, rather than in cells in general, Fawzi says.
The research was funded by the National Institutes of Health, the DEARS Foundation, and the Rhode Island Foundation.
Zachary Monahan, Veronica H Ryan, Abigail M Janke, Kathleen A Burke, Shannon N Rhoads, Gül H Zerze, Robert O’Meally, Gregory L Dignon, Alexander E Conicella, Wenwei Zheng, Robert B Best, Robert N Cole, Jeetain Mittal, Frank Shewmaker, Nicolas L Fawzi
Phosphorylation of the FUS low‐complexity domain disrupts phase separation, aggregation, and toxicity
The EMBO Journal (2017) e201696394
Image: Monahan et al./Brown