How Frogs Switch Vision To See In Murky Water

For nearly a century, scientists have puzzled over how freshwater fish and some amphibians, including frogs, can do something humans can’t.

They can easily shift their vision from marine or terrestrial environments, where the light environment is blue-green, to the waters of inland steams where mud, algae, and other particles filter out light and create an environment that shifts to the red and infrared end of the spectrum.

Now, scientists believe they have solved the mystery, says senior author Joseph Corbo, associate professor of pathology and immunology at Washington University in St. Louis:

“We’ve discovered an enzyme that switches the visual systems of some fish and amphibians and supercharges their ability to see infrared light.

For example, when salmon migrate from the ocean to inland streams, they turn on this enzyme, activating a chemical reaction that shifts the visual system, helping the fish peer more deeply into murky water.”

Vitamin A Enzyme

As it turns out, the enzyme— called Cyp27c1— is closely linked to vitamin A, long known to promote good vision, especially in low light. The enzyme converts vitamin A1 to vitamin A2, which has remarkable properties to enhance the ability to see longer wavelength light such as red and infrared light.

The findings could lead to advances in biomedical research, particularly in optogenetics, in which light is used to control the firing of neurons in the brain. Optogenetic applications currently are limited to visible light, which penetrates only the top layer of neural tissue.

Cyp27c1 enzyme

This graphic illustrates how the Cyp27c1 enzyme supercharges the ability of freshwater fish and amphibians to see red and infrared light. (J. Corbo Laboratory)

But if scientists are able to incorporate the newly discovered enzyme, they may be able to activate photosensitive neurons with infrared light, which penetrates much deeper.

“Just as the enzyme helps fish peer into murky water, it could help us peer deeper into the brain,” Corbo says.

Researchers made the discovery using zebrafish and confirmed their findings in bullfrogs, whose eyes are uniquely designed for the light environments of both air and freshwater.

Bullfrogs sit with their eyes at the water’s surface so that they can look up into the air and down into the water at the same time. Researchers found vitamin A2 and the enzyme Cyp27c1 right where they expected: in the upper half of the bullfrog’s eyes that peer down into the water, but not in the lower half which looks upward into the air.

Natural Night-vision

Furthermore, the scientists discovered that zebrafish with normal copies of the cyp27c1 gene move toward infrared light shined into a dark aquarium. But fish with disabled cyp27c1 genes continue to behave like they are in the dark, whether or not the infrared light is on.

Humans have a form of the same gene, but it is not turned on in the eye. Thus, people are not able to enhance their infrared vision in the same way fish can. To do so, they must wear night-vision goggles.

“We don’t know yet how this enzyme is utilized in the human body,” Corbo says. “But just because our eyes don’t make vitamin A2 doesn’t mean we can’t use it.”

Research on medical students in the 1940s showed that people who consume vitamin A2 have an enhanced ability to detect red and infrared light. In 2013, a group of “biohackers” successfully crowdfunded an experiment to try to extend their vision into the near-infrared spectrum by eating a diet supplemented with vitamin A2.

“I wouldn’t necessarily recommend following their dietary advice, but the concept is sound,” Corbo says.

Enright, Jennifer M. et al.
Cyp27c1 Red-Shifts the Spectral Sensitivity of Photoreceptors by Converting Vitamin A1 into A2
Current Biology DOI:

Photo: Mark Iocchelli/Flickr