Neutron analysis was recently used, by a team led by the Department of Energy’s Oak Ridge National Laboratory, to better understand a protein involved in replication of HIV, the retrovirus behind AIDS. The enzyme, called HIV-1 protease, is a key drug target for HIV and AIDS therapies.
Researchers from ORNL, Georgia State University and the Institut Laue-Langevin in France used neutron crystallography to uncover details of interactions of hydrogen bonds at the enzyme’s active site, revealing a pH-induced proton hopping mechanism that guides its activity.
Clarifying the enzyme’s structure and function at the atomic level, including the location and movement of hydrogen atoms, is essential for understanding drug resistance and guiding rational drug design.
HIV-1 protease is responsible for the maturation of virus particles into infectious HIV virions, which ultimately leads to the development of AIDS. Without effective HIV-1 protease activity, HIV virions remain non-infectious, so the disruption of HIV-1 protease activity is a key target for successful antiretroviral therapy (ART) drugs that attack the virus itself.
Hydrogen-bonding Interactions Revealed
The use of X-ray crystallography to study the structures of HIV-1 protease and drug complexes has led to the design of effective, commercially available ART drugs, but x-rays cannot determine the positions of mobile hydrogen atoms and protons. Neutron crystallography, however, can reveal these hydrogen-bonding interactions, which play a key role in how effectively a drug binds to its target.
The ORNL-ILL-Georgia State research team used neutron crystallography to probe the structure of HIV-1 protease in complex with the clinical inhibitor Darunavir.
The researchers combined neutron diffraction data from the IMAGINE instrument at ORNL’s High Flux Isotope Reactor (HFIR), and the LAD-III instrument at ILL, to uncover details of the hydrogen-bonding interactions in the active site and reveal ways to enhance drug binding and reduce drug resistance. The group also examined how the enzyme’s catalytic activity responds to changes in pH (acidity) levels.
By determining structures at different pHs, the group was able to directly observe positions of hydrogen atoms before and after a pH-induced two-proton transfer between the drug and enzyme. The proton transfer, triggered by electrostatic effects arising from proton uptake by surface residues from solution, resulted in the proton configuration that is critical for the catalytic activity.
Institut Laue-Langevin instrument scientist Matthew Blakely said:
“These results highlight that neutrons represent a superb probe to obtain structural details for proton transfer reactions in biological systems.”
ORNL instrument scientist Andrey Kovalevsky added:
“Darunavir’s structure allows it to create more hydrogen bonds with the protease active site than most drugs of its type, while the backbone of HIV-1 protease maintains its spatial conformation in the presence of mutations.
This means Darunavir-protease interaction is less likely to be disrupted by a mutation. Given these characteristics, Darunavir is an excellent therapy target to refine and therefore enhance HIV treatment.”
Direct observation of proton transfer in chemical and biological systems is challenging. Macromolecular neutron crystallography has been pivotal in providing key details regarding hydrogen bonding that were required in order to answer long-standing questions about the enzyme mechanism of this important HIV drug target.
“Moreover, we observed changes in hydrogen configurations induced by changes in protein surface charges at long distances,” said Kovalevsky. “This phenomenon may occur in other aspartic proteases, and perhaps in enzymes more generally.”
With the recent improvements that have been made, the field of macromolecular neutron crystallography is expanding, with studies addressing a variety of important biological processes from protein-folding to antibiotic resistance and proton transport across biological membranes.
Oksana Gerlits et al.
Long-Range Electrostatics-Induced Two-Proton Transfer Captured by Neutron Crystallography in an Enzyme Catalytic Site
Angewandte Chemie International Edition (2016). DOI: 10.1002/anie.201509989
Image: A 3D structure of the HIV-1 protease in cartoon representation with bound clinical drug darunavir (shown as sticks). The catalytic site contains two closely positioned aspartic acid residues. The insert depicts the hydrogen transfer reaction in the catalytic site, captured for the first time by neutron crystallography. Credit: Jill Hemman and Andrey Kovalevsky, Oak Ridge National Laboratory