An experimental targeted molecular therapy has been used to block a matrix-forming protein in heart cells damaged by heart attack, reducing levels of scarred muscle tissue and saving mouse models from heart failure.
Researchers at the Cincinnati Children’s Heart Institute report testing a manufactured peptide called pUR4 to block the fibronectin protein in human heart cells donated by heart failure patients.
The treatment prevented the human heart cells from failing and restored their function. The treatment also reduced fibrosis and improved heart function after a simulated heart attack in mice.
Fibronectin is normally a good actor in the body. It helps form a cell-supporting matrix for the body’s connective tissues, aiding tissue repair after injury.
But after a heart attack, fibronectin overreacts, it polymerizes and helps produce too much connective matrix. It also causes hyperactive production of clogged and dysfunctional cardiomyofibroblast cells that damage the heart.
The pUR4 compound is designed so it will attach to surface points on fibronectin, effectively inhibiting its effects in injured heart cells.
“Our data are a strong proof of principle and the first to show that inhibiting fibronectin polymerization preserves heart function, reduces left ventricle remodeling and limits formation of fibrotic connective tissue,"
said the study’s lead investigator Burns Blaxall, PhD, director of translational research in the Heart Institute and the Center for Translational Fibrosis Research.
Ongoing Medical Challenge
Although heart disease is the leading cause of death in the world, few effective treatment options are available for patients — many having had a previous heart attack or congenital heart disease. The pUR4 molecular treatment used in the current study is one of several compounds Blaxall and his colleagues are testing that show promise in preliminary preclinical research data.
Heart fibrosis and cardiac remodeling are medical challenges that are also faced by children with congenital heart disorders, and ongoing medical challenges requiring specialized care often follow these children into adulthood.
[caption id=“attachment_95197” align=“aligncenter” width=“680”] Human heart cells treated with a novel therapeutic peptide exhibit dramatically reduced fibrosis.
Credit: Cincinnati Children’s[/caption]
A key question in the study was verifying the results of pUR4 targeted molecular therapy in both the mouse models and human heart failure cells. In mice with simulated heart attack that as a control experiment received a placebo therapy, the animals developed significant fibrosis and heart failure.
When researchers treated mice with pUR4 for just the first seven days after heart attack, or genetically deleted fibronectin activity from the heart cells of mice, these reduced fibrosis and improved cardiac function. Treatment of human failing heart cells with pUR4 also reduced their fibrotic behavior.
Fibrocyte cells normally secrete collagen, and function to provide structural support for the heart. When over-activated this process causes thickening and fibrosis of the valve, with white tissue building up primarily on the tricuspid valve, but also occurring on the pulmonary valve.
The thickening and loss of flexibility eventually may lead to valvular dysfunction and right-sided heart failure.
The researchers emphasize it’s too early to know whether the experimental therapy in this study can one day be used to treat human heart patients clinically. Extensive additional research is needed first, including proving pUR4’s safety in larger animal models and then moving on to establish proof-of-principal effectiveness treating heart failure in those models, according to Blaxall.
Researchers also are working to refine the pUR4 peptide to enhance its capabilities for localized administration to the heart and for extended-release in patients.
Iñigo Valiente-Alandi, Sarah J. Potter, Ane M. Salvador, Allison E. Schafer, Tobias Schips, Francisco Carrillo-Salinas, Aaron M. Gibson, Michelle L. Nieman, Charles Perkins, Michelle A. Sargent, Jiuzhou Huo, John N. Lorenz, Tony DeFalco, Jeffery D. Molkentin, Pilar Alcaide and Burns C. Blaxall Inhibiting Fibronectin Attenuates Fibrosis and Improves Cardiac Function in a Model of Heart Failure Circulation. 2018;CIRCULATIONAHA.118.034609, originally published April 13, 2018
Top Image: donated fibrotic heart cells from a patient who had heart failure. The cells have an elaborate fibronectin matrix (shown in red) which causes fibrosis and heart damage. Credit: Cincinnati Children’s