E-cigarettes Can Alter Genes In Airway Immune Defense

When you smoke cigarettes, dozens of genes crucial to immune defense are altered in the epithelial cells lining the respiratory tract. Several of these changes likely raise the risk of bacterial infections, viruses, and inflammation.

Scientists at University of North Carolina School of Medicine now report that vaping electronic cigarettes alters those same genes and hundreds more that are important for immune defense in the upper airway.

Lead researcher Ilona Jaspers, professor of pediatrics, and microbiology and immunology at UNC, said:

“I was really surprised by these results. That’s why we kept going back to make sure this was accurate.”

Jasper is the paper’s senior author and director of UNC’s toxicology curriculum.

Epithelial Cell Gene Consequences

The finding suggests that inhaling the vaporized flavored liquids in e-cigarettes is not without consequences, at least on the level of epithelial cell gene expression, the critical process by which our genes give rise to proteins important for various functions in cells.

The discovery cannot yet be linked to long-term health effects of e-cigarette use or the risk of diseases usually associated with long-term cigarette smoking such as cancer, emphysema, or chronic obstructive pulmonary disease.

“We honestly do not yet know what long-term effects e-cigarettes might have on health,” said Jaspers. “I suspect that the effects of e-cigarettes will not be the same as the effects of cigarette smoking.”

So far, though, the evidence suggests that long-term e-cigarette use will not be harmless.

E-cigarettes have only been on the market in the United States since 2006, and usage skyrocketed just a few years ago. The more than 7,000 flavors available in e-cigarettes are FDA approved, though that approval process was based on data generated for oral consumption, not inhalation.

Critical For Immune Defense

To study what effects e-cigarettes have on genes that help our upper airways fight off potentially harmful pathogens, Jaspers’ lab recruited 13 non-smokers, 14 smokers, and 12 e-cigarette users.

Each participant kept a journal documenting their cigarette or e-cigarette use, and in collaboration with researchers from the University of California at San Francisco, Jaspers’ team analyzed participant urine and blood samples to confirm nicotine levels and biomarkers relevant to tobacco exposure.

After about three weeks, researchers took samples from the nasal passages of each participant to analyze the expression of genes important for immune responses.

Visually and functionally, the epithelial layers of our nasal passages are very similar to the epithelial layers in our lungs. All epithelial cells along our airways, from our noses to the tiny bronchioles deep in our lungs, need to function properly to trap and dispatch particles and pathogens so we don’t get sick.

These epithelial cells are critical for normal immune defense. Certain genes in these cells must give rise to proper amounts of proteins, which orchestrate the overall immune response.

It has long been known that cigarette smoking modifies this gene expression, which is one reason researchers think smokers are more sensitive to upper respiratory problems.

Mechanism Unknown

Using the non-smokers as the baseline comparison group, Jaspers’ team found that smoking cigarettes decreased the gene expression of 53 genes important for the immune response of epithelial cells. Using e-cigarettes decreased the gene expression of 358 genes important for immune defense – including all 53 genes implicated in the smoking group.

“We compared these genes one by one,” Jaspers said, “And we found that each gene common to both groups was suppressed more in the e-cigarette group. We currently do not know exactly how e-cigarettes do this.”

Jaspers said her lab’s findings do not mean that smoking e-cigarettes is as bad as or worse than smoking regular cigarettes.

“I think it is a mistake to try to directly compare cigarette smoking and e-cigarette use,” Jaspers said. “We shouldn’t ask ‘smoking causes cancer; do e-cigarettes cause cancer? Smoking causes emphysema; do e-cigarettes cause emphysema?'”

She said that inhaling burnt tobacco and inhaling vaporized flavored liquids are fundamentally different, and it’s more likely that e-cigarettes could induce different biological changes and play different roles in other respiratory problems.

“We know that diseases like COPD, cancer, and emphysema usually take many years to develop in smokers,” Jaspers said. “But people have not been using e-cigarettes for very long. So we don’t know yet how the effects of e-cigarette use might manifest in 10 or 15 years. We’re at the beginning of cataloging and observing what may or may not be happening.”

Next, Jaspers will study how epithelial cells in e-cigarette users respond to a flu vaccine. This, she said, could help her team measure the immune response of epithelial cells in smokers, non-smokers, and e-cigarette users.

The research was funded through the UNC Center for Tobacco Regulatory Science and Lung Health (TCORS), one of 14 such centers around the United States created through a collaboration between the U.S. Food and Drug Administration (FDA) and the National Institutes of Health (NIH).

Elizabeth Martin, Phillip W Clapp, Meghan E Rebuli, Erica A Pawlak, Ellen E Glista-Baker, Neal L Benowitz, Rebecca C Fry, Ilona Jaspers
E-cigarette use results in suppression of immune and inflammatory-response genes in nasal epithelial cells similar to cigarette smoke
American Journal of Physiology – Lung Cellular and Molecular Physiology, 2016; ajplung.00170.2016 DOI: 10.1152/ajplung.00170.2016

Image: Sophia Davidson, NIMR London/Flickr