New research could revolutionize the slow, cumbersome and expensive process of detecting the antibodies that can help with the diagnosis of infectious and auto-immune diseases such as rheumatoid arthritis and HIV. An international team of researchers have designed and synthetized a nanometer-scale DNA “machine” whose customized modifications enable it to recognize a specific target antibody.
The new approach promises to support the development of rapid, low-cost antibody detection at the point-of-care, eliminating the treatment initiation delays and increasing healthcare costs associated with current techniques.
The binding of the antibody to the DNA machine causes a structural change (or switch), which generates a light signal. The sensor does not need to be chemically activated and is rapid - acting within five minutes - enabling the targeted antibodies to be easily detected, even in complex clinical samples such as blood serum.
Said Prof. Francesco Ricci, of the University of Rome, Tor Vergata, senior co-author of the study:
“One of the advantages of our approach is that it is highly versatile. This DNA nanomachine can be in fact custom-modified so that it can detect a huge range of antibodies, this makes our platform adaptable for many different diseases”.
Added Prof. Vallée-Bélisle of the University of Montreal, the other senior co-author of the paper:
“Our modular platform provides significant advantages over existing methods for the detection of antibodies. It is rapid, does not require reagent chemicals, and may prove to be useful in a range of different applications such as point-of-care diagnostics and bioimaging”.
Said Prof. Kevin Plaxco of the University of California, Santa Barbara.
“Another nice feature of our this platform is its low-cost. The materials needed for one assay cost about 15 cents, making our approach very competitive in comparison with other quantitative approaches."
Simona Ranallo, a PhD student in the group of Prof. Ricci at the University of Rome and first-author of the paper, said:
“We are excited by these preliminary results, but we are looking forward to improve our sensing platform even more. For example, we could adapt our platform so that the signal of the nanoswitch may be read using a mobile phone. This will make our approach really available to anyone! We are working on this idea and we would like to start involving diagnostic companies."
Ranallo, S., Rossetti, M., Plaxco, K. W., Vallée-Bélisle, A. and Ricci, F. A Modular, DNA-Based Beacon for Single-Step Fluorescence Detection of Antibodies and Other Proteins Angew. Chem. Int. Ed.. doi: 10.1002/anie.201505179
Top Illustration: Marco Tripodi