Optogenetics has taken neuroscience by storm in recent years because the technique allows scientists to study the brain conveniently in animals, activating or inhibiting selected groups of neurons at the flip of a switch. Most often, scientists use a fiber optic cable to deliver light into the brain.
Researchers at Emory and Georgia Tech have developed tools that could allow neuroscientists to put aside the fiber optic cable, and use a glowing protein from coral as the light source instead.
[caption id=“attachment_21766” align=“alignleft” width=“300”] Bovine Rhodopsin: S. Jähnichen[/caption]
Biomedical engineering student Jack Tung and neurosurgeon/neuroscientist Robert Gross, MD, PhD have dubbed these tools “inhibitory luminopsins” because they inhibit neuronal activity both in response to light and to a chemical supplied from outside.
A demonstration of the luminopsins' capabilities was published September 24 in the journal Scientific Reports. The authors show that these tools enabled them to modulate neuronal firing, both in culture and in vivo, and modify the behavior of live animals.
Tung and Gross are now using inhibitory luminopsins to study ways to halt or prevent seizure activity in animals.
“We think that this approach may be particularly useful for modeling treatments for generalized seizures and seizures that involve multiple areas of the brain,” Tung says. “We’re also working on making luminopsins responsive to seizure activity: turning on the light only when it is needed, in a closed-loop feedback controlled fashion."
Limits of Fiber Optics
[caption id=“attachment_21765” align=“alignright” width=“300”] Fiber optic illuminated by Hustvedt - Template:One.[/caption]
In conventional optogenetics, scientists use genetic engineering techniques to make neurons in animals produce light-sensitive proteins called opsins.
These opsins, which come from various algae and salt-tolerant bacteria, respond to a particular wavelength of light by moving ions across the cell membrane, thus stimulating or silencing the neurons where they are expressed.
However, fiber optic cables, the usual mode of delivering the light needed to activate the opsins, have a limited reach into the brain, pose a risk of infection and can limit animals' movements.
To supply light locally and internally, the team took the enzyme luciferase from the soft coral Renilla, which glows in presence of its substrate luciferin, and fused it to an inhibitory opsin – creating a novel fusion protein termed an inhibitory luminopsin (from “luminescent opsin”).
An excitatory luminopsin has been reported by other scientists, but its properties were demonstrated only in cultured cells.
Disabling the Globus Pallidus
To show that luminopsins could modulate behavior in live animals, they injected a gene vector encoding their inhibitory luminopsin into the globus pallidus of rats, on just one side of the brain. Two weeks later, the rats were injected with luciferin.
The globus pallidus is involved in motor control, and the presence of luciferin had the effect of disabling the globus pallidus. In response to the drug amphetamine, the animals rotated preferentially in one direction, which mimicked the behavior that results when the globus pallidus is damaged on one side.
“We chose to do the rotation tests because they were simple and easy to measure,” Tung says. “We also wanted to show that expression of luminopsin could be scaled up to multiple structures in the brain."
The researchers also showed that luminopsins and luciferase together could suppress neural activity in the hippocampus, another region of the brain, in anesthetized rats.
The authors note that similar techniques have been used to stimulate selected neurons in animals with designer drugs, but conclude that luminopsins could be cleaner and easier to use, while offering scientists the choice of influencing neuronal activity with either light or an externally supplied chemical that is otherwise inert.
Jack K. Tung, Claire-Anne Gutekunst, Robert E. Gross Inhibitory luminopsins: genetically-encoded bioluminescent opsins for versatile, scalable, and hardware-independent optogenetic inhibition Scientific Reports, 2015; 5: 14366 DOI: 10.1038/srep14366
_Top Illustration: Bioluminescence from cells cultured in a multielectrode array, next to a fiber optic cable. Credit: Jack Tung _