Chemotherapy for acute myeloid leukemia could be improved by giving patients a drug currently used to treat an unrelated condition, researchers led by a team from Imperial College London have found.

Acute myeloid leukaemia (AML) is an aggressive cancer that stops healthy blood cell production. Chemotherapy is the standard treatment, but improvements are needed as the five-year survival rate in patients older than 60 is only 5-15 per cent.

Now, by studying how leukaemia cells infiltrate bone marrow, where blood cells are created, researchers made a crucial discovery.

Deferoxamine Protects Stem Cells

Studying mice and human samples, they found that certain areas in the bone marrow support blood stem cells, and when these are overtaken by leukaemia cells, these stem cells are lost and production of healthy blood is significantly reduced. This can cause anaemia, infection, and bleeding in patients, and affects the success of chemotherapy.

Most importantly, the team also discovered that Deferoxamine, a drug already approved to treat a condition known as iron overload, can protect these important bone marrow areas and allow blood stem cells to survive.

“Since the drug is already approved for human use for a different condition, we already know that it is safe. We still need to test it in the context of leukaemia and chemotherapy, but because it is already in use we can progress to clinical trials much quicker than we could with a brand new drug,"

said lead author, Dr Cristina Lo Celso from the Department of Life Sciences at Imperial.

The researchers are now hoping to team up with clinicians to begin human trials of the drug for AML. Understanding whether this drug is a viable option should take less than five years, as opposed to the 10-15 needed if an entirely new drug is developed.

Progressive Remodeling Of Endosteal Stroma

The team conducted the study by filming the invasion of leukaemia cells into bone marrow in mice. This approach allowed them see both large overviews and incredible details of the bone marrow, revealing phenomena happening deep inside the bone marrow - a view usually inaccessible to direct observation in patients.

[caption id=“attachment_93930” align=“aligncenter” width=“680”]bone marrow Healthy bone marrow (yellow) invaded by leukaemia, with blood vessels in cyan (red).
Credit: Delfim Duarte/Imperial College London[/caption]

The group discovered that one of the spaces hit particularly hard by leukaemia were special regions of blood vessels where blood stem cells reside. These are the basic blood cells that can become all other types of blood cells, including red and white, generating billions of new cells every day of our life.

For this reason, these special blood vessel regions are vital for producing new healthy blood, and their destruction by leukaemia allows the disease to progress. The loss of these vessels was confirmed in humans by studying patient tissue samples.

Improved Chemotherapy Efficiency

To see if they could protect the vessels, the team tested a drug called deferoxamine. The drug is used to treat iron overload, which can happen for example when a person receives multiple blood transfusions.

Deferoxamine has also been used in the treatment of myelodysplasia, a disease related to leukaemia where young blood stem cells do not mature into healthy blood cells. Other researchers who contributed to this project, and are now based at Imperial, Max Plank Munster, and Oxford Kennedy Institute, showed that this drug increases bone marrow vessels in aged mice.

[caption id=“attachment_93931” align=“aligncenter” width=“680”]Endosteal blood vessels Endosteal blood vessels decreased in bone marrow full of leukaemia (shown with arrows).
Credit: Delfim Duarte/Imperial College London[/caption]

Dr Lo Celso’s group now found that the drug had a protective effect on the blood vessels in AML, allowing the rescue of healthy blood stem cells. Moreover, the enhanced vessels improved the efficiency of chemotherapy.

“Our work suggests that therapies targeting these blood vessels may improve existing therapeutic regimes for AML, and perhaps other leukaemias too,"

said Delfim Duarte, a physician and PhD student who performed most of the experiments.

Delfim Duarte, et al Inhibition of Endosteal Vascular Niche Remodeling Rescues Hematopoietic Stem Cell Loss in AML Cell Stem Cell; December 21, 2017

Top Image: yellow leukaemia invading the bone marrow, dislodging red normal blood cells and destroying cyan blood vessels and blue osteoblasts. Credit: Delfim Duarte/Imperial College London

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