The amygdala is involved in making judgments about ambiguous or intense emotions in facial expressions, researchers at California Institute of Technology have found.
When looking at a face, brain cells in the amygdala fire electrical impulses or “spikes” in response. However, the role of such face cells in social cognition remains unclear.
Ralph Adolphs, Bren Professor of Psychology and Neuroscience and professor of biology, said:
“We have long known that the amygdala is important in processing emotion from faces. But now we are starting to understand that it incorporates a lot of complex information to make fairly sophisticated decisions that culminate in our judgments."
Adolphs and collaborator Ueli Rutishauser of Cedars-Sinai Medical Center in Los Angeles measured the activity of these cells, or neurons, in patients while they were shown images of faces expressing different degrees of happiness or fear. The subjects were also shown images of faces with more ambiguous or neutral emotions, such as moderate displeasure or muted happiness.
For each type of image, subjects were asked to decide whether the face looked fearful or happy. The researchers then investigated how neurons reacted to different aspects of emotions, and how the activity of the face cells related to the decision made by the subjects.
Two Amygdala Neuron Groups
The researchers found that there are two groups of neurons in the amygdala that respond to facial emotions.
One group, the emotion-tracking neurons, detects the intensity of a single specific emotion, such as happiness or fear.
For example, a happiness-signaling neuron would fire more spikes if the emotion was extreme happiness, and fewer spikes if the emotion was mild happiness. Separate groups of neurons within the emotion-tracking neurons code specifically for fear or for happiness.
The other group, the ambiguity-coding neurons, indicates the ambiguity of the perceived emotion, irrespective of the nature of that emotion.
[caption id=“attachment_90643” align=“aligncenter” width=“680”] Researchers from the Adolphs lab showed subjects faces such as these, expressing fear, neutrality, and happiness. Credit: U. Rutishauser[/caption]
Showing patients images of emotionally ambiguous faces was the key to understanding how the specialized neurons in the amygdala contribute to decision making, the researchers say. The faces were so ambiguous that a patient would sometimes judge the same image to be fearful at times and happy at other times.
The emotion-coding neurons indicated the subjective decision the patient made about the face.
First author Shuo Wang said:
“Most people are familiar with feeling that a face just looks too ambiguous to really decide what emotion the person is having. The fact that amygdala neurons signal a decision made about a face, such as which emotion it shows, gives us important insight because it shows that the amygdala is involved in making decisions rather than simply representing sensory input."
In addition to recording single cells from the amygdala, the researchers also carried out a neuroimaging study using fMRI in a separate group of participants, and additionally studied the emotion judgments of three rare subjects with lesions of the amygdala.
The lesion subjects showed an abnormally low threshold for deciding when a face was fearful, and the fMRI study also showed the specific effect of emotion intensity and ambiguity in the amygdala. The study is the first to combine so many different sources of data.
These findings also suggest a mechanistic basis for potential treatments involving the painless electrical stimulation of the amygdala, which are currently being studied in ongoing clinical trials.
“Researchers at multiple institutions are currently evaluating whether deep-brain stimulation of the amygdala is effective in treating severe cases of autism or post-traumatic stress disorder,” says Rutishauser. “Patients with severe PTSD are thought to have a hyperactive amygdala, which electrical stimulation might be able to inhibit. Our findings that amygdala neurons carry signals about the subjective percept of emotions indicates a more specific reason for why such electrical stimulation might be beneficial."
The research was supported by the Autism Science Foundation, the Simons Foundation, the Cedars-Sinai Department of Neurosurgery, an NSF CAREER award and the NIMH Conte Center.
Shuo Wang, Rongjun Yu, J. Michael Tyszka, Shanshan Zhen, Christopher Kovach, Sai Sun, Yi Huang, Rene Hurlemann, Ian B. Ross, Jeffrey M. Chung, Adam N. Mamelak, Ralph Adolphs & Ueli Rutishauser The human amygdala parametrically encodes the intensity of specific facial emotions and their categorical ambiguity Nature Communications 8, Article number: 14821 (2017) doi:10.1038/ncomms14821
Image: Alyssa L. Miller/Flickr