People who develop Parkinson’s disease before age 50 may have been born with disordered brain cells that went undetected for decades, research from Cedars-Sinai Medical Center suggests. The study reports on a drug that potentially might help correct these disease processes.
Although most Parkinson’s patients are 60 or older when they are diagnosed, about 10% are between 21 and 50 years old.
“Young-onset Parkinson’s is especially heartbreaking because it strikes people at the prime of life. This exciting new research provides hope that one day we may be able to detect and take early action to prevent this disease in at-risk individuals,"
said co-author of the study[1] Michele Tagliati, MD, director of the Movement Disorders Program, vice chair and professor in the Department of Neurology at Cedars-Sinai.
Time Tunnel
To perform the study, the research team generated special stem cells, known as induced pluripotent stem cells (iPSCs), from cells of patients with young-onset Parkinson’s disease. This process involves taking adult blood cells “back in time” to a primitive embryonic state.
These iPSCs can then produce any cell type of the human body, all genetically identical to the patient’s own cells. The team used the iPSCs to produce dopamine neurons from each patient and then cultured them in a dish and analyzed the neurons’ functions.
“Our technique gave us a window back in time to see how well the dopamine neurons might have functioned from the very start of a patient’s life,"
said the study’s senior author, Clive Svendsen, Ph.D., director of the Cedars-Sinai Board of Governors Regenerative Medicine Institute and professor of Biomedical Sciences and Medicine at Cedars-Sinai.
PEP005
The researchers detected two key abnormalities in the dopamine neurons in the dish:
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Accumulation of a protein called alpha-synuclein, which occurs in most forms of Parkinson’s disease.
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Malfunctioning lysosomes, cell structures that act as “trash cans” for the cell to break down and dispose of proteins. This malfunction could cause alpha-synuclein to build up.
“What we are seeing using this new model are the very first signs of young-onset Parkinson’s,” said Svendsen. “It appears that dopamine neurons in these individuals may continue to mishandle alpha-synuclein over a period of 20 or 30 years, causing Parkinson’s symptoms to emerge."
The investigators also used their iPSC model to test a number of drugs that might reverse the abnormalities they had observed. They found that that one drug, PEP005, which is already approved by the Food and Drug Administration for treating pre-cancers of the skin, reduced the elevated levels of alpha-synuclein in both the dopamine neurons in the dish and in laboratory mice.
The drug also countered another abnormality they found in the patients’ dopamine neurons — elevated levels of an active version of an enzyme called protein kinase C — although the role of this enzyme version in Parkinson’s is not clear.
Next Steps
Tagliati said the team plans to investigate how PEP005, currently available in gel form, might be delivered to the brain to potentially treat or prevent young-onset Parkinson’s. The team also plans more research to determine whether the abnormalities the study found in neurons of young-onset Parkinson’s patients also exist in other forms of Parkinson’s.
“This research is an outstanding example of how physicians and investigators from different disciplines join forces to produce translational science with the potential to help patients. This important work is made possible by the dual leadership of Cedars-Sinai as both a distinguished academic institution and an outstanding hospital,"
said Shlomo Melmed, MB, ChB, executive vice president of Academic Affairs and dean of the Medical Faculty at Cedars-Sinai.
[1] Laperle, A.H., Sances, S., Yucer, N. et al. iPSC modeling of young-onset Parkinson’s disease reveals a molecular signature of disease and novel therapeutic candidates. Nat Med (2020). https://doi.org/10.1038/s41591-019-0739-1